Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 cells
genotype
WT
replicate
1
chip antibody
none
cell type
S2 cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with polyclonal αgH2Av antibodies (3mL). Finally DNA was purified by standard phenol extraction. 10 ng DNA, quantified by Qubit dsDNA HS Assay Kit (Invitrogen) was used for library preparation. Endrepair, adenylation, ligation of adapters and PCR enrichment for 15 cycles was performed using TruSeqRNA Sample Prep Kit (Illumina) according to manufacturer’s recommendations. Purified libraries were quantified by Qubit dsDNA HS Assay Kit (Invitrogen) and size distribution was evaluated using Bioanalyzer DNA 1000 assay (Agilent).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
25406310
Reads aligned (%)
95.2
Duplicates removed (%)
17.0
Number of peaks
4806 (qval < 1E-05)

dm3

Number of total reads
25406310
Reads aligned (%)
95.8
Duplicates removed (%)
15.6
Number of peaks
4376 (qval < 1E-05)

Base call quality data from DBCLS SRA