Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adult
Cell type
Testis
NA
NA

Attributes by original data submitter

Sample

source_name
Testes, bam-, input
genotype/variation
bam-
tissue
Testis
age
Within 3 days of eclosion
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Testes were dissected in cold PBS, fixed, sonicated, and clarified lysates were used for ChIP with specific antibodies. Libraries were prepared with the NEXTflex ChIPseq Kit (Bioo Scientific) according to manufacturer's suggestions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
19779275
Reads aligned (%)
80.8
Duplicates removed (%)
56.8
Number of peaks
2524 (qval < 1E-05)

dm3

Number of total reads
19779275
Reads aligned (%)
82.2
Duplicates removed (%)
54.1
Number of peaks
2247 (qval < 1E-05)

Base call quality data from DBCLS SRA