Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Adult

Cell type

Testis

NA

NA

Sample

source_name

Testes, bam-, H3K4me2 ChIP

genotype/variation

bam-

tissue

Testis

age

Within 3 days of eclosion

chip antibody

H3K4me2 (Abcam, Y47)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Testes were dissected in cold PBS, fixed, sonicated, and clarified lysates were used for ChIP with specific antibodies. Libraries were prepared with the NEXTflex ChIPseq Kit (Bioo Scientific) according to manufacturer's suggestions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

20266485

Reads aligned (%)

82.5

Duplicates removed (%)

45.5

Number of peaks

8030 (qval < 1E-05)

dm3

Number of total reads

20266485

Reads aligned (%)

82.6

Duplicates removed (%)

44.2

Number of peaks

8950 (qval < 1E-05)