GSM2617027: ChIP 97L H3K36me3 Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Liver
Cell type
97L
NA
NA
Attributes by original data submitter
Sample
source_name
hepatoma cell line
cell line
97L
tissue
liver
chip antibody
H3K4me3 (human, Abcam, ab8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Methylation: Genomic DNA from tissues and cell lines was extracted using QIAamp DNA Mini Kit (Qiagen, 51306, Valencia, CA). 3μg genomic DNA was fragmented into 300-500bp by sonication. ChIP:Approximate 300 ug fixed chromatin and 5 ug antibody were used for each ChIP pull-down. RNA-seq: We extracted approximate 6 micrograms of total RNA, removed ribosomal RNA (rRNA) according to the manufacturer's instructions with minor modifications. Libraries were prepared for sequencing using standard Illumina protocols