GSM2616310: H3K4me3 GIC-387 shIDH1-98; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Neural
Cell type
GIC-387
NA
NA
Attributes by original data submitter
Sample
source_name
Glioma Initiating Cells
cell line
GIC-387
shRNA sequence
shIDH1-98 (TRCN000027298)
chip antibody
H3K4me3 (Hu et al., 2013; doi: 10.1038/nsmb.2653)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
cells per sample were fixed with 1% formaldehyde and ChIP-seq was performed according to previous studies (Chen et al., 2015; doi: 10.1016/j.cell.2015.07.042 and Chen et al. 2015; doi: 10.1101/gad.246173.114) ChIP-sequencing libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250- 450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.