Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Blood
Cell type
Peripheral blood
NA
NA

Attributes by original data submitter

Sample

source_name
peripheral blood
cell type
FACS-purified lymphocytes; short-term culture
antibody
anti-H3K4Me2, Abcam ab32356

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Murine ILC2s were obtained from Gata3-YFP/YFP mice with airway inflammation, human ILC2s from peripheral blood. Cells were FACS-sorted using specific antibodies (and YFP for murine cells) as described in detail in the manuscript. ChIPmentation: Nextera DNA library construction (as described in Schmidl et al. Nature Methods 2015); RNA-Seq: Smart-seq2library construction (as described in Picelli et al. Nature Methods (2013)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
56482890
Reads aligned (%)
97.2
Duplicates removed (%)
24.8
Number of peaks
17523 (qval < 1E-05)

hg19

Number of total reads
56482890
Reads aligned (%)
96.5
Duplicates removed (%)
25.6
Number of peaks
17402 (qval < 1E-05)

Base call quality data from DBCLS SRA