Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Lung

Cell type

Bronchoalveolar Lavage

MeSH Description

Washing out of the lungs with saline or mucolytic agents for diagnostic or therapeutic purposes. It is very useful in the diagnosis of diffuse pulmonary infiltrates in immunosuppressed patients.

Sample

source_name

bronchoalveolar lavage (BAL)

cell type

FACS-purified lymphocytes

antibody

anti-H3K4Me2, Abcam ab32356

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Murine ILC2s were obtained from Gata3-YFP/YFP mice with airway inflammation, human ILC2s from peripheral blood. Cells were FACS-sorted using specific antibodies (and YFP for murine cells) as described in detail in the manuscript. ChIPmentation: Nextera DNA library construction (as described in Schmidl et al. Nature Methods 2015); RNA-Seq: Smart-seq2library construction (as described in Picelli et al. Nature Methods (2013)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

43391446

Reads aligned (%)

98.5

Duplicates removed (%)

23.6

Number of peaks

35335 (qval < 1E-05)

mm9

Number of total reads

43391446

Reads aligned (%)

98.4

Duplicates removed (%)

23.6

Number of peaks

35418 (qval < 1E-05)