Curated Sample Data


Genome
mm9
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Lung
Cell type
Bronchoalveolar Lavage

Cell type information


MeSH Description
Washing out of the lungs with saline or mucolytic agents for diagnostic or therapeutic purposes. It is very useful in the diagnosis of diffuse pulmonary infiltrates in immunosuppressed patients.

Attributes by Original Data Submitter


source_name
bronchoalveolar lavage (BAL)
cell type
FACS-purified lymphocytes
antibody
anti-H3K4Me2, Abcam ab32356

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Murine ILC2s were obtained from Gata3-YFP/YFP mice with airway inflammation, human ILC2s from peripheral blood. Cells were FACS-sorted using specific antibodies (and YFP for murine cells) as described in detail in the manuscript. ChIPmentation: Nextera DNA library construction (as described in Schmidl et al. Nature Methods 2015); RNA-Seq: Smart-seq2library construction (as described in Picelli et al. Nature Methods (2013)

Platform Information


instrument_model
NextSeq 500

External Database Query

Logs in read processing pipeline


Number of total reads
34874978
Reads aligned (%)
89.4
Duplicates removed (%)
16.9
Number of peaks
31617 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA