Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse embryonic stem cells, Smarca4_WT, input
strain/background
129SvEv
cell type
Embryonic stem cells
passage
15-20
smarca4
Smarca4_WT
chip antibody
None
chip antibody vendor
n/a

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were obtained from sonicated nuclei crosslinked with formaldehyde, and protein-DNA complexes were isolated by immunoprecipitation. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
75565598
Reads aligned (%)
98.6
Duplicates removed (%)
17.1
Number of peaks
641 (qval < 1E-05)

mm9

Number of total reads
75565598
Reads aligned (%)
98.4
Duplicates removed (%)
17.1
Number of peaks
714 (qval < 1E-05)

Base call quality data from DBCLS SRA