Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.
Attributes by Original Data Submitter
Haematopoietic progenitor cells
C57BL/6 cebpa flox/flox
IgG: Sigma, I8140
Metadata from Sequence Read Archive
Chromatin from 100.000 preGMs or 500.000 GMPs was incubated with antibodies for histone marks (H3K4me3 and H3K27me3) or C/EBPa, respectively. The antibody-bound chromatin was captured with Protein-A sepharose beads, washed, de-cross-linked and precipitated. DNA was extracted using standard phenol-chloroform extraction Precipitated DNA were mixed with 2 ng E. Coli DNA and amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturers protocol.