Curated Sample Data


Genome
mm9
Antigen Class
Input control
Antigen
Input control
Cell type Class
Blood
Cell type
Hematopoietic Stem Cells

Cell type information


MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by Original Data Submitter


source_name
Haematopoietic progenitor cells
cell type
LSK_CebpaWT
strain
C57BL/6 cebpa flox/flox
chip-antibody
IgG: Sigma, I8140

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from 100.000 preGMs or 500.000 GMPs was incubated with antibodies for histone marks (H3K4me3 and H3K27me3) or C/EBPa, respectively. The antibody-bound chromatin was captured with Protein-A sepharose beads, washed, de-cross-linked and precipitated. DNA was extracted using standard phenol-chloroform extraction Precipitated DNA were mixed with 2 ng E. Coli DNA and amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturers protocol.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
80676851
Reads aligned (%)
38.8
Duplicates removed (%)
20.5
Number of peaks
1066 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA