Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Uterus

Cell type

SK-UT-1

Primary Tissue

Uterus

Tissue Diagnosis

Leiomyosarcoma

Sample

source_name

uterine leiomyosarcomas_SK-UT-1_FASN-expressing

cell line

SK-UT-1

tissue

uterine leiomyosarcoma

chip antibody

N/A

passage

3~5

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) were performed on SK-UT-1-FASN and SK-UT-1-EV cells using the Magna ChIP A/G kit from (Millipore, Temecula, CA) following the manufacturer’s protocol. Anti-H3K9me3 antibody was used to immunoprecipitate cross-linked and fragmented chromatin DNA. This was followed by elution of protein/DNA complexes, reversal of the cross-links, and DNA purification. Library preparation and massively parallel DNA sequencing (ChIP-seq) was performed with eluted DNA using an Illumina Hiseq 2500 sequencer by the City of Hope Integrative Genomics Core (Duarte, CA).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

38702948

Reads aligned (%)

99.3

Duplicates removed (%)

4.4

Number of peaks

983 (qval < 1E-05)

hg19

Number of total reads

38702948

Reads aligned (%)

98.3

Duplicates removed (%)

5.7

Number of peaks

1020 (qval < 1E-05)