Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
Drosohpila s2 cells
cell type
S2 cells
transfection
Actin-Gal4 and UAS-E(Pc) cDNA-GFP plasmids
chip-antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
S2 cell fixation and following ChIP were performed with ChIP-IT high sensitivity kit (#53040, Active motif) following the manufacturer’s instruction. Sonication of S2 cells were done with Bioruptor sonicator (UCD-200, diagenode) using following setting 0.5min on, 1min off for total 15min. Fragment size were around 500-600bp. ChIP grade GFP antibody (Abcam, ab290) were used to pulled down sheared chromatin. Libraries were generated using reagents provided in the Illumina TruSeq ChIP Sample Preparation Kit (IP-202-1012).

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
2506825
Reads aligned (%)
99.4
Duplicates removed (%)
14.6
Number of peaks
131 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA