Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
E14 ES cells
cell type
E14 ES cells
chip antibody
none
genotype
Control

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA were extracted using antibodies for MTF2, SUZ12, H3K27me3. RNA-Seq samples were prepared by isolating total RNA using Trizol, mRNA Isolation using Oligo-dT-coupled beads, heat fractionation, reverse transcription and second strand synthesis. Libraries were constructed of 10-50 ng of DNA following Illumina’s® protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
18904628
Reads aligned (%)
93.7
Duplicates removed (%)
10.4
Number of peaks
424 (qval < 1E-05)

mm9

Number of total reads
18904628
Reads aligned (%)
93.5
Duplicates removed (%)
10.5
Number of peaks
445 (qval < 1E-05)

Base call quality data from DBCLS SRA