Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ep300

Cell type

Cell type Class
Blood
Cell type
Th17 Cells
MeSH Description
A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Attributes by original data submitter

Sample

source_name
Th17 cells
strain
C57BL/6
tissue
Th17 differentiated in vitro for 3 days
genotype
wild type
chip antibody
p300 antibody(santa-cruz, sc-585)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted from cells by Trizol. ChIP-seq was performed from crosslinked and MNase digested nuclear lysates; protein-DNA complexes were pulled down by indicated antibodies; after reverse-crosslinking, DNA was purified by phenol-chloroform. RNA libraries were prepared by the BerryGenomics company and only mRNA was used. ChIP libraries were constructed following the protocol of NEXTflex ChIP-Seq DNA Sequencing Kit(Bioo Scientific, 5143) described in the paper.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
50585623
Reads aligned (%)
98.9
Duplicates removed (%)
54.6
Number of peaks
208 (qval < 1E-05)

mm9

Number of total reads
50585623
Reads aligned (%)
98.7
Duplicates removed (%)
54.7
Number of peaks
213 (qval < 1E-05)

Base call quality data from DBCLS SRA