Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Th17 Cells
MeSH Description
A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Attributes by original data submitter

Sample

source_name
Junb+/+CD4cre Th17 cells
time point
48 h
chip antibody
none
strain
C57Bl/6 (N5 backcross)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Th17 cells were crosslinked and the resulting sonicate was subjected to immunoprecipitation using 10μg of anti-JunB or anti-JunD. Following reverse-crosslinking, ChIP and input samples were processed to obtain purified DNA. Libraries were constructed from ChIP and Input DNA using the Kapa Hyper Prep Kit

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
43094335
Reads aligned (%)
98.5
Duplicates removed (%)
20.7
Number of peaks
522 (qval < 1E-05)

mm9

Number of total reads
43094335
Reads aligned (%)
98.2
Duplicates removed (%)
20.7
Number of peaks
569 (qval < 1E-05)

Base call quality data from DBCLS SRA