Antigen

Antigen Class

TFs and others

Antigen

Jund

Cell type

Cell type Class

Blood

Cell type

Th17 Cells

MeSH Description

A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Sample

source_name

Junb+/+CD4cre Th17 cells

time point

48 h

chip antibody

JunD (329; sc-74x)

strain

C57Bl/6 (N5 backcross)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Th17 cells were crosslinked and the resulting sonicate was subjected to immunoprecipitation using 10μg of anti-JunB or anti-JunD. Following reverse-crosslinking, ChIP and input samples were processed to obtain purified DNA. Libraries were constructed from ChIP and Input DNA using the Kapa Hyper Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

47516670

Reads aligned (%)

98.1

Duplicates removed (%)

21.8

Number of peaks

3242 (qval < 1E-05)

mm9

Number of total reads

47516670

Reads aligned (%)

97.9

Duplicates removed (%)

21.8

Number of peaks

3272 (qval < 1E-05)