GSM1139142: IgG Control RNAPII; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
MEL
Tissue
Blood
Lineage
cellLine
Description
Leukemia (K562 analog)
Attributes by original data submitter
Sample
source_name
Murine erythroleukemia (MEL) cells
cell line
non-induced C88 MEL cells
chip antibody
anti-IgG
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10 ng of ChIPped DNA was end-repaired, ligated to adapters, size selected on gel (200±25 bp range) and PCR amplified using Phusion polymerase. Cluster generation was performed using the Illumina Cluster Reagents preparation. ChIP-Seq library was prepared according to the Illumina protocol (www.illumina.com).