Antigen

Antigen Class

TFs and others

Antigen

SOX2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

H9_SOX2_AF2018

cell lines

H9

antibody

SOX2

antibody vendor

R&D (AF2018)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked with 1% formaldehyde, washed with PBS, lysed in lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl, pH 8.0), sonicated with Covaris E210 to shear DNA to 200-1500bp. After immunoprecipitation or without immunoprecipitation for input, beads were washed, RNase A and Proteinase K treated and reverse crosslinked in elution buffer (1% SDS, 0.1M NaHCO3). DNAs were column purified and subjected to standard Illumina library construction.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

12000000

Reads aligned (%)

96.0

Duplicates removed (%)

2.7

Number of peaks

823 (qval < 1E-05)

hg19

Number of total reads

12000000

Reads aligned (%)

95.2

Duplicates removed (%)

4.2

Number of peaks

1024 (qval < 1E-05)