GSM1139033: KYSE70 p63 4A4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TP63
Cell type
Cell type Class
Digestive tract
Cell type
KYSE-70
Primary Tissue
Esophagus
Tissue Diagnosis
Carcinoma Squamous Cell
Attributes by original data submitter
Sample
source_name
KYSE70_p63_4A4
cell lines
KYSE70
antibody
p63
antibody vendor
Santa-Cruz (sc-8431)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked with 1% formaldehyde, washed with PBS, lysed in lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl, pH 8.0), sonicated with Covaris E210 to shear DNA to 200-1500bp. After immunoprecipitation or without immunoprecipitation for input, beads were washed, RNase A and Proteinase K treated and reverse crosslinked in elution buffer (1% SDS, 0.1M NaHCO3). DNAs were column purified and subjected to standard Illumina library construction.