Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
E14 ES cells, control, input
cell line
E14
cell type
embryonic stem (ES) cells
treatment
DMSO
chip_antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H4K16ac ChIPs were performed according to the recommendations of the Diagenode low-cell ChIP kit (C01010072). ChIP-seq libraries were prepared using NEBNext ChIPseq library prep kit E6200L using NEB index primers 2, 4, 5, 6, 7, and 12.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
22604635
Reads aligned (%)
95.2
Duplicates removed (%)
18.9
Number of peaks
515 (qval < 1E-05)

mm9

Number of total reads
22604635
Reads aligned (%)
94.9
Duplicates removed (%)
18.9
Number of peaks
542 (qval < 1E-05)

Base call quality data from DBCLS SRA