glioblastoma stem-like cells, transduced with shControl
tumor stage
human glioblastoma multiformis
chip antibody
H3K4me3 (Millipore, 07-473)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-Seq libraries were constructed with the DNA Seq sample prep. kit (ref. IP-102-1001) from Illumina according to the manufacturer’s instructions.