Arrigoni et al. 2015. Standardizing chromatin research: a simple and universal method for ChIP-seq. Nucleic Acids Res. 2016 Apr 20;44(7):e67. doi: 10.1093/nar/gkv1495. Epub 2015 Dec 23. For ChIP-seq experiment, C2C12 cells were fixed for 6 minutes with 1 % PFA at 4 °C followed by 5 minutes blocking in 125 mM glycine. Nuclei preparation was performed using previously described NEXON protocol37, by sonicating the cells with Covaris E220 focused ultrasonicator for 2 minutes at peak power 75 W, duty factor 2 % and 200 Cycles/burst at 4 °C. Chromatin was sheared using Covaris E220 focused ultrasonicator to obtain a fragment size distribution of 100–800 bp (peak power: 140 W; Duty factor: 5 %; Cycles/burst: 200, water temperature 4 °C). Chromatin was immunopercipitated using homemade antibody directed against C-terminus of Lsd1 protein (Biogenes, 20752) as described (Dutel et al., 2014) Libraries were prepared from immunoprecipitated DNA according to standard methods. ChIP-seq libraries were sequenced using a HiSeq 2000 (Illumina) and mapped to the mm10 reference genome using bowtie 2 Langmead, B., Trapnell, C., Pop, M. & Salzberg, S.L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10, R25 (2009).