Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Erg

Cell type

Cell type Class
Blood
Cell type
Myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
ERG_TgERG_leukemia_1
antibody
ERG-1/2/3 (clone C-17, Sc354X, Santa Cruz)
strain
C57BL6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Spleen cells were fixed with 1% formaldehyde and ChIP assays were performed as previously described (Wilson NK et al., 2010 Cell Stem Cell) using polyclonal antibodies against ERG-1/2/3 (clone C-17, Sc354X, Santa Cruz) and control nonspecific rabbit IgG (I5006, Sigma). Each sample was amplified and sequenced using the Illumina 2G Genome Analyzer, following manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
39959209
Reads aligned (%)
91.7
Duplicates removed (%)
9.5
Number of peaks
10360 (qval < 1E-05)

mm9

Number of total reads
39959209
Reads aligned (%)
91.6
Duplicates removed (%)
9.6
Number of peaks
10246 (qval < 1E-05)

Base call quality data from DBCLS SRA