Antigen

Antigen Class

TFs and others

Antigen

EBNA1

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

CC

cell line

GM12878

chip antibody

anti-EBNA1 antibody 0211 (Santa Cruz, sc-57719, lot K2013)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

BZLF1 and EBNA1 ChIPs were performed as previously described (Keck et al., submitted) using 3 µg of the anti-BZLF1 antibody BZ1 (Santa Cruz, sc-53904, lot 2313) and 3 µg of the anti-EBNA1 antibody 0211 (Santa Cruz, sc-57719, lot K2013). We prepared deep sequencing libraries by adaptor-mediated amplification using Ovation Ultralow Library System V2 (NuGEN).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

33983731

Reads aligned (%)

96.4

Duplicates removed (%)

13.9

Number of peaks

1447 (qval < 1E-05)

hg19

Number of total reads

33983731

Reads aligned (%)

93.8

Duplicates removed (%)

13.9

Number of peaks

1760 (qval < 1E-05)