Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS cell line
cell type
U2OS
cell type
osteosarcoma
treatment
no treatment control 24 hr and ChIP with p53 (control for Doxorubicin-treated cells)
chip antibody
p53 DO-1 (Santa Cruz, sc-126)

Sequenced DNA Library

library_name
ERROR: cannot retrieve data from NCBI: too many requests
library_strategy
ERROR: cannot retrieve data from NCBI: too many requests
library_source
ERROR: cannot retrieve data from NCBI: too many requests
library_selection
ERROR: cannot retrieve data from NCBI: too many requests
library_construction_protocol
ERROR: cannot retrieve data from NCBI: too many requests

Sequencing Platform

instrument_model
ERROR: cannot retrieve data from NCBI: too many requests
cycle_sequence
ERROR: cannot retrieve data from NCBI: too many requests
cycle_count
ERROR: cannot retrieve data from NCBI: too many requests
flow_sequence
ERROR: cannot retrieve data from NCBI: too many requests
flow_count
ERROR: cannot retrieve data from NCBI: too many requests
key_sequence
ERROR: cannot retrieve data from NCBI: too many requests

hg19

Number of total reads
21509088
Reads aligned (%)
81.3
Duplicates removed (%)
4.6
Number of peaks
840 (qval < 1E-05)

hg38

Number of total reads
21509088
Reads aligned (%)
83.5
Duplicates removed (%)
2.9
Number of peaks
522 (qval < 1E-05)

Base call quality data from DBCLS SRA