GSM2587055: THP-1, GABPA 24 h EtOH; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GABPA
Cell type
Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
human monocytic cell line THP-1
cell line
THP-1
cell type
monocytic leukemia cells
chip antibody
GABP-a (Santa Cruz sc-28312 X)
treatment protocol
24 h EtOH
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Proteins were crosslinked with formaldehyde to DNA, then GABPA-bound fragments were pulled down by chromatin immunoprecipitation ChIP-seq library was prepared from 5-10 ng ChIP DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) per manufacturer's protocol