Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GABPA

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
human monocytic cell line THP-1
cell line
THP-1
cell type
monocytic leukemia cells
chip antibody
GABP-a (Santa Cruz sc-28312 X)
treatment protocol
24 h EtOH

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Proteins were crosslinked with formaldehyde to DNA, then GABPA-bound fragments were pulled down by chromatin immunoprecipitation ChIP-seq library was prepared from 5-10 ng ChIP DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) per manufacturer's protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
25673262
Reads aligned (%)
88.1
Duplicates removed (%)
10.1
Number of peaks
1172 (qval < 1E-05)

hg38

Number of total reads
25673262
Reads aligned (%)
90.4
Duplicates removed (%)
8.6
Number of peaks
1111 (qval < 1E-05)

Base call quality data from DBCLS SRA