GSM1133649: LY1 H3K4ME3 JQ1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Blood
Cell type
OCI-LY1
NA
NA
Attributes by original data submitter
Sample
source_name
Diffuse Large B-Cell Lymphoma
chip antibody
H3K4me3
chip antibody catalog number
07-473
chip antibody manufacturer
Millipore
cell line
OC-LY1
time
24 hours
concentration
500nM
treatment
JQ1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.