GSM2584781: U251 V5-OGDH cells input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Neural
Cell type
U-251 MG
Primary Tissue
Brain
Tissue Diagnosis
Astrocytoma
Attributes by original data submitter
Sample
source_name
U251
antibody
input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The libraries were amplified by using three cycles of PCR and then assessed for quality by using a Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies, Ankeny, IA) and quantified by using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).