Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF516

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Breast cancer cell line
cell line
MCF-7
cell type
Breast cancer cell line
passages
15-18
chip antibody
ZNF516
chip antibody vendor
Bethyl

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ZNF516-DNA complexes were isolated with antibody. The chromatin DNA precipitated by polyclonal antibodies against ZNF516 or none for input control. The DNA was purified with the Qiagen PCR purification kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
18795924
Reads aligned (%)
96.0
Duplicates removed (%)
19.9
Number of peaks
794 (qval < 1E-05)

hg19

Number of total reads
18795924
Reads aligned (%)
95.2
Duplicates removed (%)
20.2
Number of peaks
319 (qval < 1E-05)

Base call quality data from DBCLS SRA