Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL6

Cell type

Cell type Class
Digestive tract
Cell type
DLD-1
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
colorectal adenocarcinoma cell line
cell line
DLD-1
passage
10-14
disease state
Dukes' type C, colorectal adenocarcinoma
antibody
rabbit polyclonal anti-BCL6 (N-3) (sc-858 X)
treatment
transfected with pEGFP-C1199Tiam1 and treated with vehicle (RAC1/PAK1 activation)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Beads were thoroughly washed and co-precipitated DNA was purified with the IPure Kit (Diagenode), after cross-link reversion Library construction for ChIP sequencing was performed using the TruSeq DNA sample prep kit from Illumina and following manufacturer's instruction.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
7789142
Reads aligned (%)
94.3
Duplicates removed (%)
1.7
Number of peaks
354 (qval < 1E-05)

hg38

Number of total reads
7789142
Reads aligned (%)
95.3
Duplicates removed (%)
1.3
Number of peaks
231 (qval < 1E-05)

Base call quality data from DBCLS SRA