GSM2582002: HAX-11 Inhibitor-STAT5; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
STAT5A
Cell type
Cell type Class
Digestive tract
Cell type
DLD-1
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
colorectal adenocarcinoma cell line
cell line
DLD-1
passage
10-14
disease state
Dukes' type C, colorectal adenocarcinoma
antibody
rabbit polyclonal anti-STAT5 (C-17) (sc-835 X)
treatment
transfected with pEGFP-C1199Tiam1 and treated with 20 microM EHT 1864 (RAC1/PAK1 inhibition)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Beads were thoroughly washed and co-precipitated DNA was purified with the IPure Kit (Diagenode), after cross-link reversion Library construction for ChIP sequencing was performed using the TruSeq DNA sample prep kit from Illumina and following manufacturer's instruction.