Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT5A

Cell type

Cell type Class
Digestive tract
Cell type
DLD-1
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
colorectal adenocarcinoma cell line
cell line
DLD-1
passage
10-14
disease state
Dukes' type C, colorectal adenocarcinoma
antibody
rabbit polyclonal anti-STAT5 (C-17) (sc-835 X)
treatment
transfected with pEGFP-C1199Tiam1 and treated with 20 microM EHT 1864 (RAC1/PAK1 inhibition)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Beads were thoroughly washed and co-precipitated DNA was purified with the IPure Kit (Diagenode), after cross-link reversion Library construction for ChIP sequencing was performed using the TruSeq DNA sample prep kit from Illumina and following manufacturer's instruction.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
12070212
Reads aligned (%)
94.5
Duplicates removed (%)
2.2
Number of peaks
545 (qval < 1E-05)

hg38

Number of total reads
12070212
Reads aligned (%)
95.8
Duplicates removed (%)
1.5
Number of peaks
385 (qval < 1E-05)

Base call quality data from DBCLS SRA