Antigen

Antigen Class

TFs and others

Antigen

STAT5A

Cell type

Cell type Class

Digestive tract

Cell type

DLD-1

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

colorectal adenocarcinoma cell line

cell line

DLD-1

passage

10-14

disease state

Dukes' type C, colorectal adenocarcinoma

antibody

rabbit polyclonal anti-STAT5 (C-17) (sc-835 X)

treatment

transfected with empty vector

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Beads were thoroughly washed and co-precipitated DNA was purified with the IPure Kit (Diagenode), after cross-link reversion Library construction for ChIP sequencing was performed using the TruSeq DNA sample prep kit from Illumina and following manufacturer's instruction.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

9883308

Reads aligned (%)

97.8

Duplicates removed (%)

1.5

Number of peaks

280 (qval < 1E-05)

hg19

Number of total reads

9883308

Reads aligned (%)

97.1

Duplicates removed (%)

2.0

Number of peaks

420 (qval < 1E-05)