The series of cells in the red blood cell lineage at various stages of differentiation.
Attributes by original data submitter
Sample
source_name
Ter119+ Erythroid cells
strain
C57BL6
tissue
Phenylhydrazine treated spleen
antibody
anti_H3K4me3, Abcam ChIP Grade (ab8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
A single cell suspension was made by gently dissociating the spleen of a phenyhydrazine treated mouse and passing it through a 70um filter. Ter119 selection was performed by staining the cells with phycoerythrin conjugated anti-ter119 antibodies (BD biosciences). The cells were subsequently washed and then incubated with MACS anti-phycoerythrin beads. Cells were then separated using an auto-MACS (Miltenyi Biotec). NEBnext ultra library prep kit