Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Digestive tract

Cell type

MKN-7

Primary Tissue

Stomach

Tissue Diagnosis

Adenocarcinoma Tubular

Sample

source_name

MKN7_EB1

cell line

MKN7

cell type

Gastric cancer cell line

infected with

epstein-barr virus (EBV)

chip antibody

H3K4me1_ChIP (abcam, ab8895, lot GR114265-2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA complexes were isolated. FAIRE-seq libraries were prepared according to Illumina's instructions. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Preparation Set for Illumina (New England Biolabs) following the manufacturer’s protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

24691404

Reads aligned (%)

99.2

Duplicates removed (%)

13.3

Number of peaks

2514 (qval < 1E-05)

hg19

Number of total reads

24691404

Reads aligned (%)

98.8

Duplicates removed (%)

14.1

Number of peaks

2572 (qval < 1E-05)