Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Digestive tract
Cell type
MKN-7
Primary Tissue
Stomach
Tissue Diagnosis
Adenocarcinoma Tubular

Attributes by original data submitter

Sample

source_name
MKN7_wt
cell line
MKN7
cell type
Gastric cancer cell line
infected with
none (control)
chip antibody
H3K4me3 (Active Motif, 39159, lot 01609004)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA complexes were isolated. ChIP-seq libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
39414814
Reads aligned (%)
98.9
Duplicates removed (%)
6.2
Number of peaks
25469 (qval < 1E-05)

hg19

Number of total reads
39414814
Reads aligned (%)
98.1
Duplicates removed (%)
7.5
Number of peaks
25487 (qval < 1E-05)

Base call quality data from DBCLS SRA