Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cardiovascular
Cell type
Heart Ventricles
MeSH Description
The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.

Attributes by original data submitter

Sample

source_name
cardiac ventricles
genotype
alphaMHC Cre recombinase positive, beta-catenin exon3 deleted-stabilized beta-catenin (transgenic)
strain
C57BL/6
chip-seq antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For each IP, ventricles from atleast two murine hearts were pooled, washed in PBS, minced finely and fixed in 1.3% Formaldehyde for 20 min. The nuclei from tissue extracts were sonicated for 45 cycles to obtain the fragmented chromatin. Chromatin extracts containing DNA fragments with an average size of 300 bp were immunoprecipitated using 2µg/IP of different antibodies. ChIP-seq library preparation was performed using NEBNext Ultra DNA library prep kit for Illumina (E7370) as per manual’s instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
46269731
Reads aligned (%)
95.8
Duplicates removed (%)
18.3
Number of peaks
475 (qval < 1E-05)

mm9

Number of total reads
46269731
Reads aligned (%)
95.6
Duplicates removed (%)
18.3
Number of peaks
483 (qval < 1E-05)

Base call quality data from DBCLS SRA