Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Adult
Cell type
Ovary
NA
NA

Attributes by original data submitter

Sample

source_name
Adult ovaries
genotype
w, moon_FS1{delta}1/moon_FS2{delta}28; ;
developmental stage
Adult
tissue
ovary
chip antibody
anti-RNA Pol II (8WG16, Abcam, ab817)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysis and sonication of formaldehyde-crosslinked ovaries NEBNext® Ultra™ DNA Library Prep Kit for Illumina® Ovaries were hand-dissected into ice-cold PBS

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm6

Number of total reads
31600235
Reads aligned (%)
89.7
Duplicates removed (%)
32.9
Number of peaks
7152 (qval < 1E-05)

dm3

Number of total reads
31600235
Reads aligned (%)
89.7
Duplicates removed (%)
32.2
Number of peaks
7331 (qval < 1E-05)

Base call quality data from DBCLS SRA