Antigen

Antigen Class

TFs and others

Antigen

KLF4

Cell type

Cell type Class

Neural

Cell type

Glioblastoma

MeSH Description

A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.

Sample

source_name

Human glioblastoma cells

tissue

Brain

cell type

Human glioblastoma cells

passage

in vitro for a long time

chip antibody

KLF4

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted for RNA-seq, genomic DNA was extracted for ChIP-seq and WGBS Library was constructed by microarray facility at Johns Hopkins University using commercially available kits (illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

44604882

Reads aligned (%)

97.0

Duplicates removed (%)

5.8

Number of peaks

1676 (qval < 1E-05)

hg19

Number of total reads

44604882

Reads aligned (%)

95.6

Duplicates removed (%)

7.3

Number of peaks

1079 (qval < 1E-05)