Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KLF4

Cell type

Cell type Class
Neural
Cell type
Glioblastoma
MeSH Description
A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.

Attributes by original data submitter

Sample

source_name
Human glioblastoma cells
tissue
Brain
cell type
Human glioblastoma cells
passage
in vitro for a long time
chip antibody
KLF4

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted for RNA-seq, genomic DNA was extracted for ChIP-seq and WGBS Library was constructed by microarray facility at Johns Hopkins University using commercially available kits (illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16126183
Reads aligned (%)
89.8
Duplicates removed (%)
4.0
Number of peaks
705 (qval < 1E-05)

hg38

Number of total reads
16126183
Reads aligned (%)
91.8
Duplicates removed (%)
2.8
Number of peaks
685 (qval < 1E-05)

Base call quality data from DBCLS SRA