Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
POU5F1

Cell type

Cell type Class
Lung
Cell type
MRC-5
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
MRC5 Day 5 after OSvK rep1
chip antibody
Oct4 (Abcam ab19857 Lot: GR187600-1)
cell line
MRC5 fibroblast
protocol
Oct4, Sox2-VP16 and Klf4 addition
time
5 days
passage
8

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
See ChIP-Endo Protocol.pdf

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
10272534
Reads aligned (%)
85.7
Duplicates removed (%)
7.0
Number of peaks
213 (qval < 1E-05)

hg19

Number of total reads
10272534
Reads aligned (%)
84.5
Duplicates removed (%)
7.1
Number of peaks
207 (qval < 1E-05)

Base call quality data from DBCLS SRA