Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Lung
Cell type
MRC-5
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
MRC5 Day 5 after OSvK rep1
chip antibody
H3K4me3 (Abcam ab8580 Lot: GR240214-2)
cell line
MRC5 fibroblast
protocol
Oct4, Sox2-VP16 and Klf4 addition
time
5 days
passage
8

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
See ChIP-Endo Protocol.pdf

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
9485819
Reads aligned (%)
91.8
Duplicates removed (%)
3.8
Number of peaks
2046 (qval < 1E-05)

hg19

Number of total reads
9485819
Reads aligned (%)
90.2
Duplicates removed (%)
3.8
Number of peaks
2034 (qval < 1E-05)

Base call quality data from DBCLS SRA