Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231 clonal cell line expressing dCas9-BFP-KRAB construct
cell line
MDA-MB-231
genotype
wild type
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
described in doi:10.1016/j.cell.2011.09.057 (2011) NEB UltraII NEBnext ultra-II kit (NEB, #E7645)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
23233919
Reads aligned (%)
97.4
Duplicates removed (%)
5.0
Number of peaks
711 (qval < 1E-05)

hg19

Number of total reads
23233919
Reads aligned (%)
96.5
Duplicates removed (%)
5.2
Number of peaks
403 (qval < 1E-05)

Base call quality data from DBCLS SRA