Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SOX2

Cell type

Cell type Class
Lung
Cell type
Lung cancer cell line
NA
NA

Attributes by original data submitter

Sample

source_name
Lung cancer cell line
tissue
non-small-lung cancer cells from Hong Kong patient
antibody
SOX2(abcam, ab59776)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and TF-Chromatin Complexes were isolated with SOX2 antibody. Libraries were prepared by the company(BGI, HongKong) according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21324511
Reads aligned (%)
78.1
Duplicates removed (%)
17.3
Number of peaks
2299 (qval < 1E-05)

hg38

Number of total reads
21324511
Reads aligned (%)
84.1
Duplicates removed (%)
13.3
Number of peaks
2711 (qval < 1E-05)

Base call quality data from DBCLS SRA