Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NCAPH2

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293 cells
cell line
HEK293
cell type
human embryonic kidney cells
passages
10-13 passages
chip antibody
NCAPH2 (Bethyl: A302-275A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were obtained from sonicated nuclei and chromatin was isolated with NCPAH2 antibody. ChIP-seq libraries were prepared with Illumina's TruSeq DNA sample preparation kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
21412668
Reads aligned (%)
93.0
Duplicates removed (%)
5.2
Number of peaks
484 (qval < 1E-05)

hg38

Number of total reads
21412668
Reads aligned (%)
93.7
Duplicates removed (%)
4.9
Number of peaks
293 (qval < 1E-05)

Base call quality data from DBCLS SRA