Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ARID5B

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat cells
cell type
Jurkat cells
chip antibody
ARID5B antibody (HPA015037, Sigma)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Jurkat cells were cross-linked with formaldehyde for 10 min. Lysates were clarified from sonicated nuclei and ARID5B-DNA complexes were isolated with antibody. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. Library construction and sequencing by Illumina Hiseq 4000 were performed by BGI Tech Solutions Co., Limited (Hongkong).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
43881594
Reads aligned (%)
78.5
Duplicates removed (%)
17.7
Number of peaks
1808 (qval < 1E-05)

hg38

Number of total reads
43881594
Reads aligned (%)
80.8
Duplicates removed (%)
16.1
Number of peaks
3163 (qval < 1E-05)

Base call quality data from DBCLS SRA