Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat DMSO input
cell line
JURKAT
cell type
human T cell leukemia cells
treatment
DMSO
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H2BUb ChIP: 10 million cells were used for the ChIP and precipitated using antibody against human H2BUb (Cell Signaling Technology, 5546S). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
38591909
Reads aligned (%)
98.8
Duplicates removed (%)
5.5
Number of peaks
757 (qval < 1E-05)

hg19

Number of total reads
38591909
Reads aligned (%)
98.0
Duplicates removed (%)
6.8
Number of peaks
806 (qval < 1E-05)

Base call quality data from DBCLS SRA