Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat USP7i9 input
cell line
JURKAT
cell type
human T cell leukemia cells
treatment
USP7i9
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H2AUb ChIP: 10 million cells were used for the ChIP and precipitated using antibody against human H2AUb (Cell Signaling Technology, 8240S). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
28865548
Reads aligned (%)
98.9
Duplicates removed (%)
4.5
Number of peaks
584 (qval < 1E-05)

hg19

Number of total reads
28865548
Reads aligned (%)
98.1
Duplicates removed (%)
5.8
Number of peaks
670 (qval < 1E-05)

Base call quality data from DBCLS SRA