GSM2564532: Sequencing analysis of input in Jurkat cells treated with DMSO (control for H3K27me3 ChIP and H2AUb ChIP); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
Jurkat DMSO input
cell line
JURKAT
cell type
human T cell leukemia cells
treatment
DMSO
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H2AUb ChIP: 10 million cells were used for the ChIP and precipitated using antibody against human H2AUb (Cell Signaling Technology, 8240S). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.