Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K79me2

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat DMSO H3K79me2
cell line
JURKAT
cell type
human T cell leukemia cells
treatment
DMSO
chip antibody
H3K79me2 (homemade, Ali Shilatifard lab)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H3K79me2 ChIP: 10 million cells were used for the ChIP and precipitated using antibody against human H3K79me2 (homemade, Ali Shilatifard lab). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
32016868
Reads aligned (%)
98.0
Duplicates removed (%)
7.0
Number of peaks
357 (qval < 1E-05)

hg19

Number of total reads
32016868
Reads aligned (%)
97.2
Duplicates removed (%)
8.5
Number of peaks
294 (qval < 1E-05)

Base call quality data from DBCLS SRA