Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
USP7

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat USP7
cell line
JURKAT
cell type
human T cell leukemia cells
treatment
untreated
chip antibody
anti-USP7 (Bethyl, catalog# A300-033A, lot# Lot A300-033A-7)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
USP7 ChIP: 10 million cells were used for the ChIP and precipitated using antibody against human USP7 (Bethyl, A300-033A). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
17501896
Reads aligned (%)
92.4
Duplicates removed (%)
5.7
Number of peaks
24582 (qval < 1E-05)

hg38

Number of total reads
17501896
Reads aligned (%)
94.0
Duplicates removed (%)
4.7
Number of peaks
24659 (qval < 1E-05)

Base call quality data from DBCLS SRA